Massenspektrometrische in-situ-Analytik für die Problembereiche Gesundheit, Umwelt, Klima und Sichherheit

Ein Forschungs-Schwerpunkt im LOEWE-Programm des Hessischen Ministeriums für Wissenschaft und Kunst

Bisher sind im Rahmen von Ambiprobe folgende Arbeiten veröffentlicht worden:

  1. Identification of collagen IV derived danger/alarm signals in insect immunity by nanoLC-FTICR MS. B. Altincicek, A. Berisha, K. Mukherjee, B. Spengler, A. Römpp, A. Vilcinskas, Biol. Chem. 2009,390, 1303-1311. (Projekte C3, D2)
  2. Monitoring of Paclitaxel, Taxine B and 10-Deacethylbaccatin III in Taxus baccata L. by nano LC-FTMS and NMR Spectroscopy. A. Ghassempour, H. Rezadoost, A. Mashouf, HY Aboul-Enein, B. Spengler, A. Roempp, Chromatographia 2010, 72, 833-839. (Projekt D2)
  3. AP-MALDI Imaging of neuropeptides in mouse pituitary gland with 5 µm spatial resolution and high mass accuracy. S. Guenther, A. Römpp, W. Kummer, B. Spengler, Int. J. Mass Spectrom. 2010, online. (Projekte A3, C3, D2)
  4. Isolation and sequence analysis of peptides from the skin secretion of the middle east tree frog Hyla savignyi. M. Langsdorf, A. Ghassempour, A. Roempp, B. Spengler, Analytical and Bioanalytical Chemistry 2010, 398, 2853-2865. (Projekte C3, D2)
  5. Characterization of a peptide family from the skin secretion of the middle east tree frog Hyla savignyi by composition-based de novo sequencing. M. Langsdorf, A. Ghassempour, A. Roempp, B. Spengler, Rapid Comm. Mass Spectrom. 2010, 24, 2885-2899. (Projekte C3, D2)
  6. Matrix vapour deposition / recrystallization and dedicated spray preparation for high-resolution Scanning Microprobe MALDI Imaging mass spectrometry (SMALDI-MS) of tissue and single cells.W. Bouschen, O. Schulz, D. Eikel, B. Spengler, Rapid Comm. Mass Spectrom. 2010, 24, 355-364. (Projekt A3)
  7. Laser spot size and laser power dependence of ion formation in high resolution MALDI imaging. S. Guenther, M. Koestler, O. Schulz, B. Spengler, Int. J. Mass Spectrom., 2010, 294, 7-15. (Projekt A3)
  8. Histology by mass spectrometry: label-free tissue characterization obtained from high accuracy bioanalytical imaging. A. Roempp, S. Guenther, Y. Schober, O. Schulz, Z. Takats, W. Kummer, B. Spengler, Angewandte Chemie Int. Ed. 2010, 49, 3834-3838. (Projekte A3, D2)
  9. Geschärfte Sinne für Gesundheit, Umwelt, Klima und Sicherheit. Spiegel der Forschung, JLU, Sonderheft Spitzenforschung 2010, 27, 64-69. (Alle Projekte)
  10. Imaging mzML (imzML) - a common data format for imaging mass spectrometry. Römpp, A., T. Schramm, A. Hester, I. Klinkert, R. Heeren, M. Stöckli, J.-P. Both, A. Brunelle and B. Spengler, Data Mining in Proteomics. New York, Humana Press (book chapter) 2010, 205-224. (Projekte C3, D2)
  11. mzML - a Community Standard for Mass Spectrometry Data. Martens, L., M. Chambers, M. Sturm, D. Kessner, F. Levander, J. Shofstahl, W. H. Tang, A. Rompp, S. Neumann, A. D. Pizarro, L. Montecchi-Palazzi, N. Tasman, M. Coleman, F. Reisinger, P. Souda, H. Hermjakob, P.-A. Binz and E. W. Deutsch, Molecular & Cellular Proteomics 2010, 10.1074/mcp.R110.000133. (Projekte C3, D2)
  12. Identification of Biological Tissues by Rapid Evaporative Ionization Mass Spectrometry. Julia Balog, T. Szaniszlo, K.-C. Schaefer, J. Denes, A. Lopata, L. Godorhazy, D. Szalay, L. Balogh, L. Sasi-Szabo, M. Toth and Z. Takats, Anal. Chem. 2010,82, 7343-7350. (Projekt C3)
  13. Antimicrobial peptides: the ancient arm of the human immune system. J. A. Vilcinskas, Virulence 2010, 1, 440-464. (Projekt A2)
  14. Coevolution between pathogen-derived proteinases and proteinase inhibitors of host insects. A. Vilcinskas, Virulence 2010, 1, 206-214. (Projekt A2)
  15. In Situ, Real-Time Identification of Biological Tissues by Ultraviolet and Infrared Laser Desorption Ionization Mass Spectrometry. K.-C. Schäfer, T. Szaniszlo, S. Günther, J. Balog, J. Denes, M. K.,Balazs B. Dezso., M. Toth, B. Spengler, Zoltan Takats, Anal Chem 2011, 83, 1632-1640. (Projekte A1, A3, C3)
  16. Mass spectrometry imaging with high resolution in mass and space (HR2 MSI) for reliable investigation of drug compound distributions on the cellular level. A. Römpp, S. Guenther, Z. Takats, B. Spengler, Analytical and Bioanalytical Chemistry 2011, 401, 65-73. (Projekte A3, C3, D2)
  17. Analysis of Cyclotides in Viola ignobilis by Nano Liquid Chromatography Fourier Transform Mass Spectrometry. H. Hashempour, A. Ghassempour, N. L. Daly, B. Spengler and A. Römpp, Protein & Peptide Letters, 2011, 18, 747-752. (Projekt D2)
  18. Secondary Metabolites Released by The Burying Beetle Nicrophorus vespilloides: Chemical Analyses and Possible Ecological Functions. T. Degenkolb, R.-A. Düring, A. Vilcinskas, J. Chem. Ecol. 2011, 37, 724-735. (Projekt A2)
  19. Silicon-(Thio)urea Lewis-Acid Catalysis. R. Hrdina, C. E. Müller, R. C. Wende, K. M. Lippert, M. Benassi, B. Spengler, and P. R. Schreiner, J. Am. Chem. Soc. 2011, 133, 7624-7627. (Projekte C3, D2)
  20. Characterization of vertical aerosol fluxes by single particle mass spectrometry for micrometeorological analysis. E. Gelhausen, K.-P. Hinz, A. Schmidt, B. Spengler,  Atmospheric Research 2011, 102, 49-56. (Projekte B1, C1, C3)
  21. Protein identification by accurate mass MALDI imaging of tryptic peptides. Y. Schober, T. Schramm, B. Spengler, A. Römpp, Rapid Comm. in Mass Spectrom. 2011, 25, 2475-2483. (Projekte C3, D2)
  22. Characterization of surgical aerosols by the compact single-particle mass spectrometer LAMPAS 3. K.-P. Hinz, E. Gelhausen, K.-C. Schäfer, Z. Takats, B. Spengler, Analytical and Bioanalytical Chemistry 2011, 401, 3165-3172. (Projekt C1)
  23. AP-MALDI imaging of neuropeptides in mouse pituitary gland with 5 um spatial resolution and high mass accuracy. S. Guenther, A. Römpp, W. Kummer, B. Spengler,International Journal of Mass Spectrometry2011, 305, 228-23.(Projekt D2)
  24. Fast quantitative determination of melamine and its derivatives by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. A. Arnold, T. N. Arrey, M. Karas and M. Persike. Rapid Commun. Mass Spectrom., 2011,  25, 2844-2850. (Projekt D1)
  25. Biosynthesis of the Defensive Alkaloid (Z)-3-(2-Methyl-1-butenyl)pyridine in Stenus similis Beetle A. Schierling, M. Schott, K. Dettner, K. Seifer.  Journal of Natural Products, 2011, 74, 2231-2234.
  26. Real Time Analysis of Brain Tissue by Direct Combination of Ultrasonic Surgical Aspiration and Sonic Spray Mass Spectrometry.  K.-C. Schäfer, J. Balog, T. Szaniszló, D. Szalay, G. Mezey, J. Dénes, L. Bognár, M. Oertel, and Z. Takáts.  Analytical Chemistry 2011, 83, 7729-7735.
  27. Electrospray Post-Ionization Mass Spectrometry of Electrosurgical Aerosols.  S. Guenther, K.-C. Schaefer, J. Balog, J. Dénes, T. Majoros, K. Albrecht, M. Tóth, B. Spengler, Z. Takáts.  Journal of the American Society for Mass Spectrometry 2011, 22, 2082-2089.
  28. Laboratory characterization of metal-oxide sensors intended for in-situ analyses of pheromones ─ SOMMSA approach.  C. Wehrenfennig, M. Schott, T. Gasch, T. Sauerwald,  R.- A. Düring, A. Vilcinskas, C.-D. Kohl. Physica Status Solidi A, 2012, 209. 935-939.
  29. Mass Spectrometry of Atmospheric Aerosols. K.-P. Hinz.  Book chapter in: Comprehensive Environmental Mass Spectrometry 2012,  A. Lebedev, Ed,  ILM Publications, UK.
  30. Mass Spectrometry Imaging. B. Spengler. Book chapter in: Comprehensive Environmental Mass Spectrometry 2012, A. Lebedev, Ed, ILM Publications, UK.
  31. Effects of Unripened Cheese Supplements on Lipid and Antioxidant Status in Hypercholesterolemic SD Rats.  B.-Y. Seo, B. Spengler, A. Römpp, Y. Schober, Y. C. Yoon, E. Park.  J Korean Soc Food Sci Nutr. 2012, 41, 65-72.  
  32. Characterization of bioactive peptides derived by enzymatic hydrolysis of whey protein concentrate. Y. Schober, S. H. Yoo, H. D.Paik, E.J. Park, B. Spengler, A. Römpp, H.M. Jayaprakasha, Y. C: Yoo. Milchwissenschaft 2012, 67. 55-57. 
  33. Reactive oxygen species affect ATP hydrolysis by targeting a highly conserved amino acid cluster in the thylakoid ATP synthase γ subunit. Buchert F, Schober Y, Römpp A, Richter ML, Forreiter C Biochimica et Biophysica Acta (BBA) - Bioenergetics2012, 1817: 2038-2048.
  34. imzML - a common data format for the flexible exchange and processing of mass spectrometry imaging data. Schramm, T., A. Hester, I. Klinkert, J.-P. Both, R. Heeren, A. Brunelle, O. Laprevote, M.-F. Robbe, M. Stöckli, B. Spengler and A. Römpp. Journal of Proteomics 2012, 5106-5110.
  35. High Resolution MALDI imaging of tryptic peptides directly from tissue. Schober, Y., Guenther, S., Spengler, B., Römpp, A.  Rapid Communications in Mass Spectrometry  2012, 1141-1146.  
  36. Analysis of Wastewater Samples by Direct Combination of Thin-Film Microextraction and Desorption Electrospray Ionization Mass Spectrometry. Strittmatter, N., R.-A. Düring, Z. Takáts. Analyst 2012, 137, 4037-4044.    
  37. Single Cell Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging. Schober, Y., S. Guenther, B. Spengler and A. Römpp. Analytical Chemistry, 2012, 84:6293-6297.
  38. Improving the precision of quantitative bottom-up proteomics based on stable isotope labeled proteins. A. Konopka, M. E. Boehm, M. Rohmer, D. Baeumlisberger, M. Karas, W.-D. Lehmann. Anal Bioanal Chem 2012, accepted.


  1. Portable Electroantennographic Device. M. Schott.  Book chapter in Yellow Biotechnology 2012.
  2. One secretion  to defeat them all? - The chemical defense of three dermapteran species.  T.Gasch, R.-A. Düring A. Vilcinskas.  Journal of chemical ecology 2012.
  3. Increasing the Sensitivity of Volatile Detection in-situ: A New Portable Device to Advance the Mating Disruption Strategy in Plant Protection.  M. Schott, C. Wehrenfennig, T.Gasch, R.-A. Düring A. Vilcinskas.  Chemical Senses 2012.
  4. Ion-optical design of a high-performance multiple-reflection time-of-flight mass spectrometer and isobar separator.  M.Yavor, T.Dickel, W.R.Plaß.  Int. J. Mass Spectrom 2012.
  5. A high-resolution multiple-reflection time-of-flight mass spectrometer for research with exotic nuclei.  T. Dickel, W.R.Plaß, C.Scheidenberger, et al.  Nucl. Instr. Meth. A. 2012.
  6. Complete H/D Exchange under Ambient Conditions combined with ESI-MS/MS for Improving Small Molecule and Peptide Identification.  S. Tittebrand, B. Spengler, W.-D. Lehmann, 2012.